Human Dehydroepiandrosterone, DHEA ELISA Kit

Dehydroepiandrosterone (DHEA), also known as androstenolone or as prasterone (INN), is an endogenous steroid hormone. It is one of the most abundant circulating steroids in humans, in whom it is produced in the adrenal glands, the gonads, and the brain, where it functions as a metabolic intermediate in the biosynthesis of the androgen and estrogen sex steroids. However, DHEA also has a variety of potential biological effects in its own right, binding to an array of nuclear and cell surface receptors, and acting as a neurosteroid and neurotrophin. DHEA is also used orally as a medication and dietary supplement, to restore or increase DHEA and DHEA sulfate (DHEA-S) levels.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Dehydroepiandrosterone, DHEA ELISA Kit has high sensitivity and excellent specificity for detection of Human DHEA. No significant cross-reactivity or interference between Human DHEA and analogues was observed.

This Human Dehydroepiandrosterone, DHEA ELISA Kit employs a two-site sandwich ELISA to quantitate DHEA in samples. An antibody specific for DHEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHEA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHEA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHEA bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.