Biotinylated Human Gastrin-17 Protein, Fc, Avitag™ (MALS verified)

Gastrin is a peptide hormone that stimulates secretion of gastric acid (HCl) by the parietal cells of the stomach and aids in gastric motility. It is released by G cells in the pyloric antrum of the stomach, duodenum, and the pancreas. Gastrin binds to cholecystokinin B receptors to stimulate the release of histamines in enterochromaffin-like cells, and it induces the insertion of K+/H+ ATPase pumps into the apical membrane of parietal cells (which in turn increases H+ release into the stomach cavity) . Its release is stimulated by peptides in the lumen of the stomach.

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 30.3 kDa. The protein migrates as 34-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Immobilized Anti-Gastrin-17 antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human Gastrin-17, Fc,Avitag (Cat. No. GA7-H82F4) with a linear range of 0.1-2.5 ng/mL (QC tested).