What is the catalog number for Human Lactate dehydrogenase A (LDHA) ELISA Kit?

The catalog number for Human Lactate dehydrogenase A (LDHA) ELISA Kit is KTE61842.

What sizes are available for Human Lactate dehydrogenase A (LDHA) ELISA Kit?

Human Lactate dehydrogenase A (LDHA) ELISA Kit is available in the following size(s): 48 Tests, 96 Tests, 5x 96 Tests, 50x 96 Tests.

How many pack sizes does Human Lactate dehydrogenase A (LDHA) ELISA Kit come in?

Human Lactate dehydrogenase A (LDHA) ELISA Kit is available in 4 different pack sizes.

Is Human Lactate dehydrogenase A (LDHA) ELISA Kit in stock?

Human Lactate dehydrogenase A (LDHA) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

Human Lactate dehydrogenase A (LDHA) ELISA Kit

This Human Lactate dehydrogenase A (LDHA) ELISA Kit employs a two-site sandwich ELISA to quantitate LDHA.

Product Specifications

Background

This protein catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

CAS Number

7732-18-5

Product Name Alternative

LDHA; GSD11; LDH1; LDHM; PIG19; LDH muscle subunit; lactate dehydrogenase M; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59

Gene ID

3939

Accession Number

P00338

Reactivity

Human

Tag

LDHA

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

Please inquire

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Human Lactate dehydrogenase A (LDHA) ELISA Kit has high sensitivity and excellent specificity for detection of Human LDHA. No significant cross-reactivity or interference between Human LDHA and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

This Human Lactate dehydrogenase A (LDHA) ELISA Kit employs a two-site sandwich ELISA to quantitate LDHA in samples. An antibody specific for LDHA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLDHA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

LDHA; GSD11; LDH1; LDHM; PIG19; LDH muscle subunit; lactate dehydrogenase M; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.