Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit

The deduced 487-amino acid human and mouse MBOAT5 proteins contain 11 predicted transmembrane domains and share 88% amino acid identity. Human MBOAT5 shares 35% amino acid identity with Drosophila nessy.The deduced protein contains 10 putative transmembrane domains and a C-terminal endoplasmic reticulum (ER) retention motif (KKxx) . RT-PCR detected ubiquitous Lpcat3 expression, with highest level in testis. Lpcat3 colocalized with an ER marker protein in transfected Chinese hamster ovary cells. Knockdown of Lpcat3 in mouse melanoma cells with small interfering RNA significantly reduced cellular acyltransferase activity toward endogenous lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylserine.

LPCAT3; C3F; MBOAT5; OACT5; nessy; O-acyltransferase (membrane bound) domain containing 5; membrane bound O-acyltransferase domain containing 5; putative protein similar to nessy

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Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPCAT3. No significant cross-reactivity or interference between Human LPCAT3 and analogues was observed.

This Human Lysophospholipid acyltransferase 5 (LPCAT3) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT3 in samples. An antibody specific for LPCAT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPCAT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

LPCAT3; C3F; MBOAT5; OACT5; nessy; O-acyltransferase (membrane bound) domain containing 5; membrane bound O-acyltransferase domain containing 5; putative protein similar to nessy

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.