Human Latrophilin-3 (LPHN3) ELISA Kit

Latrophilin-3 is a member of the latrophilin subfamily of G-protein coupled receptors (GPCR) . Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species, endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. Latrophilin 3forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85)

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Human Latrophilin-3 (LPHN3) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPHN3. No significant cross-reactivity or interference between Human LPHN3 and analogues was observed.

This Human Latrophilin-3 (LPHN3) ELISA Kit employs a two-site sandwich ELISA to quantitate LPHN3 in samples. An antibody specific for LPHN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPHN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPHN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPHN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.