Human Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2) ELISA Kit

MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1. The predicted 211-amino acid MAD2B protein shares 24% and 26% sequence identity with yeast Mad2 and human MAD2L1, respectively, in the conserved regions. RT-PCR analysis revealed that both human MAD2 homologs were expressed at similar high levels in a panel of cell lines. Further binding analyses determined that the interaction of MAD2L2 with ADAM9 is mediated through a proline-rich SH3-ligand domain of ADAM9. Northern blot analysis detected 1.35-kb MAD2L3 transcripts in all tissues tested, with highest expression in testis.

MAD2L2; RP3-330O12.4; MAD2B; REV7; MAD2 (mitotic arrest deficient; yeast; homolog) -like 2; MAD2 homolog; OTTHUMP00000002273; mitotic arrest deficient homolog-like 2

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Human Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAD2L2. No significant cross-reactivity or interference between Human MAD2L2 and analogues was observed.

This Human Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAD2L2 in samples. An antibody specific for MAD2L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAD2L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAD2L2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAD2L2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

MAD2L2; RP3-330O12.4; MAD2B; REV7; MAD2 (mitotic arrest deficient; yeast; homolog) -like 2; MAD2 homolog; OTTHUMP00000002273; mitotic arrest deficient homolog-like 2

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.