Human MAP kinase-activating death domain protein (MADD) ELISA Kit

Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. This protein is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene.

MADD; DENN; FLJ35600; FLJ36300; IG20; KIAA0358; RAB3GEP; MAP-kinase activating death domain-containing protein; OTTHUMP00000197132; OTTHUMP00000197133; OTTHUMP00000197138; OTTHUMP00000197139; Rab3 GDP/GTP exch

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human MAP kinase-activating death domain protein (MADD) ELISA Kit has high sensitivity and excellent specificity for detection of Human MADD. No significant cross-reactivity or interference between Human MADD and analogues was observed.

This Human MAP kinase-activating death domain protein (MADD) ELISA Kit employs a two-site sandwich ELISA to quantitate MADD in samples. An antibody specific for MADD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMADD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MADD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MADD bound in the initial step. The color development is stopped and the intensity of the color is measured.

MADD; DENN; FLJ35600; FLJ36300; IG20; KIAA0358; RAB3GEP; MAP-kinase activating death domain-containing protein; OTTHUMP00000197132; OTTHUMP00000197133; OTTHUMP00000197138; OTTHUMP00000197139; Rab3 GDP/GTP exch

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.