Human Protein maelstrom homolog (MAEL) ELISA Kit

By excision of a P element insertion in the 5-prime UTR of the Drosophila maelstrom gene, Findley et al. (2003) isolated mael (M391), a allele of Drosophila maelstrom. They used database analysis to identify the human MAEL homolog. Confocal microscopy localized Drosophila maelstrom primarily to nuage, highly abundant particles within germline cells, and also to the nucleus and cytoplasm of germline cells. Nuclear shuttling assays showed that Drosophila maelstrom is transported between the cytoplasm and nucleus.By phenotypic characterization of Drosophila maelstrom mutants, which displayed axial patterning defects and other polarity defects, Findley et al. (2005) defined maelstrom as a spindle-class gene that affects Vasa modification.

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Human Protein maelstrom homolog (MAEL) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAEL. No significant cross-reactivity or interference between Human MAEL and analogues was observed.

This Human Protein maelstrom homolog (MAEL) ELISA Kit employs a two-site sandwich ELISA to quantitate MAEL in samples. An antibody specific for MAEL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAEL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAEL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAEL bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.