Human Mitogen-activated protein kinase 13 (MAPK13) ELISA Kit

Mitogen Activated Protein Kinase 13 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Mitogen-activated protein kinase 13 (MAPK13) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPK13. No significant cross-reactivity or interference between Human MAPK13 and analogues was observed.

This Human Mitogen-activated protein kinase 13 (MAPK13) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK13 in samples. An antibody specific for MAPK13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.