Human Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit

Target of rapamycin complex 2 subunit MAPKAP1 is a protein similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. The deduced 522-amino acid protein has a nuclear localization signal, 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. Comparison of SIN1 with homologs from various species revealed a short, highly conserved region, designated Box1, located within a larger conserved domain, designated CRIM (conserved region in middle) .

MAPKAP1; RP11-269P11.1; JC310; MGC2745; MIP1; SIN1; SIN1b; SIN1g; MEKK2-interacting protein 1; OTTHUMP00000022141; SAPK-interacting protein 1; ras inhibitor MGC2745; stress-activated map kinase interacting prote

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Human Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPKAP1. No significant cross-reactivity or interference between Human MAPKAP1 and analogues was observed.

This Human Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAP1 in samples. An antibody specific for MAPKAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPKAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

MAPKAP1; RP11-269P11.1; JC310; MGC2745; MIP1; SIN1; SIN1b; SIN1g; MEKK2-interacting protein 1; OTTHUMP00000022141; SAPK-interacting protein 1; ras inhibitor MGC2745; stress-activated map kinase interacting prote

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.