Human MAP kinase-activated protein kinase 2 (MAPKAPK2) ELISA Kit

MAP kinase-activated protein kinase 2 is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.The cDNA sequence revealed the following features (in 5-prime to 3-prime order) : a proline-rich region containing 2 putative SH3-binding sites, a kinase catalytic domain, a threonine residue phosphorylated by MAP kinase, and a nuclear localization signal.

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Human MAP kinase-activated protein kinase 2 (MAPKAPK2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPKAPK2. No significant cross-reactivity or interference between Human MAPKAPK2 and analogues was observed.

This Human MAP kinase-activated protein kinase 2 (MAPKAPK2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAPK2 in samples. An antibody specific for MAPKAPK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPKAPK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAPK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAPK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.