Human Ragulator complex protein LAMTOR3 (MAPKSP1) ELISA Kit

Mitogen-activated protein kinase scaffold protein 1 is an enzyme identified as an interacting protein that binds specifically to MAP kinase kinase MAP2K1/MEK1 and to MAP kinase MAPK2/ERK1. This protein enhances the activation of MAPK2, and thus is thought to function as an adaptor to enhance the efficiency of the MAP kinase cascade. When overexpressed in cultured cells, MP1 enhanced activation of ERK1 and activation of a reporter driven by the transcription factor ELK1. Expression of MP1 in cells increased binding of ERK1 to MEK1. MP1 apparently functions as an adaptor to enhance the efficiency of the MAP kinase cascade. The mTORC1 kinase promotes growth in response to growth factors, energy levels, and amino acids, and its activity is often deregulated in disease.

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Human Ragulator complex protein LAMTOR3 (MAPKSP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPKSP1. No significant cross-reactivity or interference between Human MAPKSP1 and analogues was observed.

This Human Ragulator complex protein LAMTOR3 (MAPKSP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKSP1 in samples. An antibody specific for MAPKSP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPKSP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKSP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKSP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.