Human Microtubule-associated protein RP/EB family member 2 (MAPRE2) ELISA Kit

MAPRE2 shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. The deduced 327-amino acid protein has significant homology with EB1 family proteins. Northern blot analysis detected a 2.6-kb transcript in T cells activated by 2 signals (i.e., cell surface antigen (s) and/or cytokine) and also in lymphocyte tumor cell lines. Immunoprecipitation analysis indicated that RP1 associates with full-length but not C terminus-deleted APC.

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Human Microtubule-associated protein RP/EB family member 2 (MAPRE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPRE2. No significant cross-reactivity or interference between Human MAPRE2 and analogues was observed.

This Human Microtubule-associated protein RP/EB family member 2 (MAPRE2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPRE2 in samples. An antibody specific for MAPRE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPRE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPRE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPRE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.