Human Myristoylated alanine-rich C-kinase substrate (MARCKS) ELISA Kit

MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. MARCKS is a filamentous actin crosslinking protein, with activity that is inhibited by PKC-mediated phosphorylation and by binding to calcium-calmodulin. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and protein kinase C signal transduction pathways in the regulation of the actin cytoskeleton.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Myristoylated alanine-rich C-kinase substrate (MARCKS) ELISA Kit has high sensitivity and excellent specificity for detection of Human MARCKS. No significant cross-reactivity or interference between Human MARCKS and analogues was observed.

This Human Myristoylated alanine-rich C-kinase substrate (MARCKS) ELISA Kit employs a two-site sandwich ELISA to quantitate MARCKS in samples. An antibody specific for MARCKS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMARCKS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MARCKS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MARCKS bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.