Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2) ELISA Kit

The deduced 1,265-amino acid protein shares significant similarity with mouse Mast205. RT-PCR ELISA detected variable MAST2 expression in all tissues examined. The deduced 1,734-amino acid protein has a calculated molecular mass of 190.5 kD. It had an apparent molecular mass of 205 kD by SDS-PAGE. Mast205 contains a central serine/threonine kinase catalytic domain, which includes an ATP-binding consensus sequence, and several potential phosphorylation sites. Northern blot analysis of mouse tissues detected Mast205 expression only in testis. Mast205 expression increased during postnatal testicular development coincident with meiosis/spermiogenesis. Immunofluorescence microscopy showed Mast205 associated with mouse spermatid manchette.

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Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAST2. No significant cross-reactivity or interference between Human MAST2 and analogues was observed.

This Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAST2 in samples. An antibody specific for MAST2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAST2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAST2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.