Human Microtubule-associated serine/threonine-protein kinase 4 (MAST4) ELISA Kit

MAST4 Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 5 isoforms of the human protein are produced by alternative splicing. Human protein kinases make up a large superfamily of homologous proteins, which are related by virtue of their kinase domains.MAST4 locates on human chromosome 5q13. The MAST4 cDNA is 7587 base pairs in length and encodes a putative protein of 2435 amino acids which contains a serine/threonine kinase domain and a PDZ domain. MAST4 protein has 64%, 63%, 59% and 39% identical aminoacid residues with MAST1, MAST2, MAST3 and MASTL respectively. RT-PCR analysis revealed relatively high expression level of MAST4 in most normal human tissues, with an exception of in testis, small intestine, colon and peripheral blood leukocyte.

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Human Microtubule-associated serine/threonine-protein kinase 4 (MAST4) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAST4. No significant cross-reactivity or interference between Human MAST4 and analogues was observed.

This Human Microtubule-associated serine/threonine-protein kinase 4 (MAST4) ELISA Kit employs a two-site sandwich ELISA to quantitate MAST4 in samples. An antibody specific for MAST4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAST4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAST4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.