Human S-adenosylmethionine synthase isoform type-2 (MAT2A) ELISA Kit

Methionine adenosyltransferase (EC 2.5.1.6) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the major methyl donor for many of the transmethylation reactions in the body.Three forms of MAT have been identified in mammalian tissues. MAT I and MAT III, which are encoded by the single-copy MAT1A gene, represent tetramers and dimers, respectively, formed from identical alpha-1 subunits and are synthesized primarily in the liver. The MAT2A promoter is highly GC rich, especially in the first 300 bp. The promoter contains several potential SP1-, v-myb-, and GATA-binding sites, as well as potential binding sites for C/EBP, HSF2, c-myb, and STATx.MAT2A promoter can efficiently drive expression from a reporter gene in both Jurkat and 293 cells.

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Human S-adenosylmethionine synthase isoform type-2 (MAT2A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAT2A. No significant cross-reactivity or interference between Human MAT2A and analogues was observed.

This Human S-adenosylmethionine synthase isoform type-2 (MAT2A) ELISA Kit employs a two-site sandwich ELISA to quantitate MAT2A in samples. An antibody specific for MAT2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAT2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAT2A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAT2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.