Human Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit

These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK) and IKKB (IKBKB), which activate NFKB , or TBK1 and IKKE (IKBKE), which activate IRF3. Activated IRF3 and NFKB induce transcription of IFNB (IFNB1) . For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1) . For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005) .Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level) . Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.

MAVS; CARDIF; DKFZp547C224; DKFZp666M015; FLJ27482; FLJ35386; FLJ38051; FLJ41962; IPS-1; IPS1; KIAA1271; MGC3260; VISA; CARD adapter inducing interferon-beta; CARD adaptor inducing IFN-beta; IFN-B promoter

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAVS. No significant cross-reactivity or interference between Human MAVS and analogues was observed.

This Human Mitochondrial antiviral-signaling protein (MAVS) ELISA Kit employs a two-site sandwich ELISA to quantitate MAVS in samples. An antibody specific for MAVS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAVS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAVS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAVS bound in the initial step. The color development is stopped and the intensity of the color is measured.

MAVS; CARDIF; DKFZp547C224; DKFZp666M015; FLJ27482; FLJ35386; FLJ38051; FLJ41962; IPS-1; IPS1; KIAA1271; MGC3260; VISA; CARD adapter inducing interferon-beta; CARD adaptor inducing IFN-beta; IFN-B promoter

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.