Human Melanocyte-stimulating hormone receptor (MC1R/MSHR) ELISA Kit

TUBB3 expression in several human cell lines, but not in a paclitaxel-resistant cell line. RNA interference revealed that HIF1A mediated hypoxia-induced TUBB3 expression. Chromatin immunoprecipitation analysis showed that HIF1A bound an HIF1A-binding site in the 5-prime flanking region of the TUBB3 gene. Methylation of an enhancer in the 3-prime flanking region abolished hypoxia-induced TUBB3 expression. Much lower Tubb3 levels were detected in all other adult mouse tissues examined except brain, where expression was even lower. Chromatin immunoprecipitation analysis showed that HIF1A bound an HIF1A-binding site in the 5-prime flanking region of the TUBB3 gene. Methylation of an enhancer in the 3-prime flanking region abolished hypoxia-induced TUBB3 expression.

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Human Melanocyte-stimulating hormone receptor (MC1R/MSHR) ELISA Kit has high sensitivity and excellent specificity for detection of Human MC1R. No significant cross-reactivity or interference between Human MC1R and analogues was observed.

This Human Melanocyte-stimulating hormone receptor (MC1R/MSHR) ELISA Kit employs a two-site sandwich ELISA to quantitate MC1R in samples. An antibody specific for MC1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMC1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MC1R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MC1R bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.