Human E3 ubiquitin-protein ligase Mdm2 (MDM2) ELISA Kit

MDM2 encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. MDM2 is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for MDM2 on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

MDM2; HDMX; MGC5370; MGC71221; hdm2; Mdm2; transformed 3T3 cell double minute 2; p53 binding protein; OTTHUMP00000183495; double minute 2; human homolog of; p53-binding protein; mouse double minute 2 homolog

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human E3 ubiquitin-protein ligase Mdm2 (MDM2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MDM2. No significant cross-reactivity or interference between Human MDM2 and analogues was observed.

This Human E3 ubiquitin-protein ligase Mdm2 (MDM2) ELISA Kit employs a two-site sandwich ELISA to quantitate MDM2 in samples. An antibody specific for MDM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

MDM2; HDMX; MGC5370; MGC71221; hdm2; Mdm2; transformed 3T3 cell double minute 2; p53 binding protein; OTTHUMP00000183495; double minute 2; human homolog of; p53-binding protein; mouse double minute 2 homolog

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.