Human Mediator of RNA polymerase II transcription subunit 13-like (MED13L) ELISA Kit

Mediator of RNA polymerase II transcription subunit 13-like encoded by MED13L is a subunit of the Mediator complex, a large complex of proteins that functions as a transcriptional coactivator for most RNA polymerase II-transcribed genes. Mediator of RNA polymerase II transcription subunit 13-like is involved in early development of the heart and brain. Defects in MED13L are a cause of transposition of the great arteries, dextro-looped (DTGA) .

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Mediator of RNA polymerase II transcription subunit 13-like (MED13L) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED13L. No significant cross-reactivity or interference between Human MED13L and analogues was observed.

This Human Mediator of RNA polymerase II transcription subunit 13-like (MED13L) ELISA Kit employs a two-site sandwich ELISA to quantitate MED13L in samples. An antibody specific for MED13L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED13L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED13L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED13L bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.