Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Human Mediator of RNA polymerase II transcription subunit 18 (MED18) ELISA Kit

This Human Mediator of RNA polymerase II transcription subunit 18 (MED18) ELISA Kit employs a two-site sandwich ELISA to quantitate MED18.

Product Specifications

Background

MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II

CAS Number

7732-18-5

Product Name Alternative

MED18; FLJ20045; p28b; TRAP/mediator complex subunit p28b; mediator of RNA polymerase transcription; subunit 18 homolog

Gene ID

54797

Accession Number

Q9BUE0

Reactivity

Human

Tag

MED18

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

Please inquire

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Human Mediator of RNA polymerase II transcription subunit 18 (MED18) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED18. No significant cross-reactivity or interference between Human MED18 and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

This Human Mediator of RNA polymerase II transcription subunit 18 (MED18) ELISA Kit employs a two-site sandwich ELISA to quantitate MED18 in samples. An antibody specific for MED18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED18 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

MED18; FLJ20045; p28b; TRAP/mediator complex subunit p28b; mediator of RNA polymerase transcription; subunit 18 homolog

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Accession Number URL

http://www.uniprot.org/uniprot/Q9BUE0

Calibration Range

Please inquire

Available Sizes

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

MART-1 / Melan-A (M2-7C10), CF594 conjugate, 0.1mg/mL
BNC940009-100 1x 100 µL

MART-1 / Melan-A (M2-7C10), CF594 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD71 (66IG10), CF640R conjugate, 0.1mg/mL
BNC400871-100 1x 100 µL

CD71 (66IG10), CF640R conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD53 (161-2), CF647 conjugate, 0.1mg/mL
BNC470324-100 1x 100 µL

CD53 (161-2), CF647 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) (VU-11D1), CF740 conjugate, 0.1mg/mL
BNC740965-100 1x 100 µL

MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) (VU-11D1), CF740 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD10 (CB-CALLA), CF740 conjugate, 0.1mg/mL
BNC740146-100 1x 100 µL

CD10 (CB-CALLA), CF740 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD3e (B-B12), 0.2mg/mL
BNUB1052-100 1x 100 µL

CD3e (B-B12), 0.2mg/mL

Sign In for Pricing
View Details