Human Protein MEMO1 (MEMO1) ELISA Kit

MEMO1 (Mediator Of Cell Motility 1) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Signaling by ERBB2. May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Is required for breast carcinoma cell migration.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Protein MEMO1 (MEMO1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MEMO1. No significant cross-reactivity or interference between Human MEMO1 and analogues was observed.

This Human Protein MEMO1 (MEMO1) ELISA Kit employs a two-site sandwich ELISA to quantitate MEMO1 in samples. An antibody specific for MEMO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEMO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEMO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEMO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.