Human Matrix extracellular phosphoglycoprotein (MEPE) ELISA Kit

MEPE encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants.

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Human Matrix extracellular phosphoglycoprotein (MEPE) ELISA Kit has high sensitivity and excellent specificity for detection of Human MEPE. No significant cross-reactivity or interference between Human MEPE and analogues was observed.

This Human Matrix extracellular phosphoglycoprotein (MEPE) ELISA Kit employs a two-site sandwich ELISA to quantitate MEPE in samples. An antibody specific for MEPE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEPE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEPE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEPE bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.