Human Mesoderm posterior protein 2 (MESP2) ELISA Kit

MESP2 encodes a member of the bHLH family of transcription factors and plays a key role in defining the rostrocaudal patterning of somites via interactions with multiple Notch signaling pathways. MESP2 is expressed in the anterior presomitic mesoderm and is downregulated immediately after the formation of segmented somites. MESP2 also plays a role in the formation of epithelial somitic mesoderm and cardiac mesoderm. Mutations in the MESP2 gene cause autosomal recessive spondylocostal dystosis 2 (SCD02) .

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Human Mesoderm posterior protein 2 (MESP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MESP2. No significant cross-reactivity or interference between Human MESP2 and analogues was observed.

This Human Mesoderm posterior protein 2 (MESP2) ELISA Kit employs a two-site sandwich ELISA to quantitate MESP2 in samples. An antibody specific for MESP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMESP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MESP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MESP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.