Human Probable E3 ubiquitin-protein ligase MID2 (MID2) ELISA Kit

Probable E3 ubiquitin-protein ligase MID2 encoded by MID2 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of MIDA results in two transcript variants encoding different isoforms.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Probable E3 ubiquitin-protein ligase MID2 (MID2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MID2. No significant cross-reactivity or interference between Human MID2 and analogues was observed.

This Human Probable E3 ubiquitin-protein ligase MID2 (MID2) ELISA Kit employs a two-site sandwich ELISA to quantitate MID2 in samples. An antibody specific for MID2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMID2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MID2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MID2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.