Human Matrix metalloproteinase-27 (MMP27) ELISA Kit

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Matrix metalloproteinase-27 (MMP27) ELISA Kit has high sensitivity and excellent specificity for detection of Human MMP27. No significant cross-reactivity or interference between Human MMP27 and analogues was observed.

This Human Matrix metalloproteinase-27 (MMP27) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP27 in samples. An antibody specific for MMP27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP27 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP27 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.