Human MMS19 nucleotide excision repair protein homolog (MMS19) ELISA Kit

MMS19 (MMS19 Homolog, Cytosolic Iron-Sulfur Assembly Component) is a Protein Coding gene. Among its related pathways are Metabolism and Cytosolic iron-sulfur cluster assembly. GO annotations related to this gene include binding and protein binding, bridging. Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER) and RNA polymerase II (POL II) transcription. As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD. Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery.

MMS19; RP11-452K12.5; FLJ34167; FLJ95146; MET18; MGC99604; MMS19L; hMMS19; MET18 homolog; MMS19 (MET18 S. cerevisiae) -like; MMS19 nucleotide excision repair homolog; MMS19-like (MET18 homolog; S. cerevisiae)

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Human MMS19 nucleotide excision repair protein homolog (MMS19) ELISA Kit has high sensitivity and excellent specificity for detection of Human MMS19. No significant cross-reactivity or interference between Human MMS19 and analogues was observed.

This Human MMS19 nucleotide excision repair protein homolog (MMS19) ELISA Kit employs a two-site sandwich ELISA to quantitate MMS19 in samples. An antibody specific for MMS19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMS19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMS19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMS19 bound in the initial step. The color development is stopped and the intensity of the color is measured.

MMS19; RP11-452K12.5; FLJ34167; FLJ95146; MET18; MGC99604; MMS19L; hMMS19; MET18 homolog; MMS19 (MET18 S. cerevisiae) -like; MMS19 nucleotide excision repair homolog; MMS19-like (MET18 homolog; S. cerevisiae)

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.