Human CDK-activating kinase assembly factor MAT1 (MNAT1) ELISA Kit

CDK-activating kinase assembly factor MAT1 encoded by MNAT1, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for MNAT1.

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Human CDK-activating kinase assembly factor MAT1 (MNAT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MNAT1. No significant cross-reactivity or interference between Human MNAT1 and analogues was observed.

This Human CDK-activating kinase assembly factor MAT1 (MNAT1) ELISA Kit employs a two-site sandwich ELISA to quantitate MNAT1 in samples. An antibody specific for MNAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMNAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MNAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MNAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.