Human Mps one binder kinase activator-like 1A (MOBKL1A) ELISA Kit

Mps one binder kinase activator-like 1A encoded by MOBKL1A is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Three transcript variants encoding different isoforms have been found for MOBKL1A.

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Human Mps one binder kinase activator-like 1A (MOBKL1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MOBKL1A. No significant cross-reactivity or interference between Human MOBKL1A and analogues was observed.

This Human Mps one binder kinase activator-like 1A (MOBKL1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MOBKL1A in samples. An antibody specific for MOBKL1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMOBKL1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MOBKL1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MOBKL1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.