Human U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) ELISA Kit

MPHOSPH10 encodes a protein that is phosphorylated during mitosis. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. The protein associates with the U3 small nucleolar ribonucleoprotein 60-80S complexes and may be involved in pre-rRNA processing.

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Human U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPHOSPH10. No significant cross-reactivity or interference between Human MPHOSPH10 and analogues was observed.

This Human U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) ELISA Kit employs a two-site sandwich ELISA to quantitate MPHOSPH10 in samples. An antibody specific for MPHOSPH10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPHOSPH10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPHOSPH10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPHOSPH10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.