Human M-phase phosphoprotein 6 (MPHOSPH6) ELISA Kit

MPHOSPH6 (M-Phase Phosphoprotein 6) is a Protein Coding gene. Among its related pathways are rRNA processing in the nucleus and cytosol and Deadenylation-dependent mRNA decay. GO annotations related to this gene include RNA binding. RNA-binding protein that associates with the RNA exosome complex. Involved in the 3-processing of the 7S pre-RNA to the mature 5.8S rRNA and may play a role in recruiting the RNA exosome complex to pre-rRNA; this function may include C1D.

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Human M-phase phosphoprotein 6 (MPHOSPH6) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPHOSPH6. No significant cross-reactivity or interference between Human MPHOSPH6 and analogues was observed.

This Human M-phase phosphoprotein 6 (MPHOSPH6) ELISA Kit employs a two-site sandwich ELISA to quantitate MPHOSPH6 in samples. An antibody specific for MPHOSPH6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPHOSPH6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPHOSPH6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPHOSPH6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.