Human MAGUK p55 subfamily member 5 (MPP5) ELISA Kit

MPP5 encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. membrane palmitoylated protein 5 participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289) . Multiple transcript variants encoding different isoforms have been found for MPP5.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human MAGUK p55 subfamily member 5 (MPP5) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPP5. No significant cross-reactivity or interference between Human MPP5 and analogues was observed.

This Human MAGUK p55 subfamily member 5 (MPP5) ELISA Kit employs a two-site sandwich ELISA to quantitate MPP5 in samples. An antibody specific for MPP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPP5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.