Human Metallophosphoesterase domain-containing protein 1 (MPPED1) ELISA Kit

MPPED1 (Metallophosphoesterase Domain Containing 1) is a Protein Coding gene. GO annotations related to this gene include hydrolase activity. An important paralog of this gene is MPPED2. May have metallophosphoesterase activity (in vitro) .

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Human Metallophosphoesterase domain-containing protein 1 (MPPED1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPPED1. No significant cross-reactivity or interference between Human MPPED1 and analogues was observed.

This Human Metallophosphoesterase domain-containing protein 1 (MPPED1) ELISA Kit employs a two-site sandwich ELISA to quantitate MPPED1 in samples. An antibody specific for MPPED1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPPED1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPPED1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPPED1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.