Human 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit

3-mercaptopyruvate sulfurtransferase encoded by MPST catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU) . Alternatively spliced transcript variants encoding same or different isoforms have been identified for MPST.

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Human 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPST. No significant cross-reactivity or interference between Human MPST and analogues was observed.

This Human 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit employs a two-site sandwich ELISA to quantitate MPST in samples. An antibody specific for MPST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPST bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.