Human Protein Mpv17 (MPV17) ELISA Kit

MPV17 encodes a mitochondrial inner membrane protein that is implicated in the metabolism of reactive oxygen species. Mutations in MPV17 have been associated with the hepatocerebral form of mitochondrial DNA depletion syndrome (MDDS) .

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Human Protein Mpv17 (MPV17) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPV17. No significant cross-reactivity or interference between Human MPV17 and analogues was observed.

This Human Protein Mpv17 (MPV17) ELISA Kit employs a two-site sandwich ELISA to quantitate MPV17 in samples. An antibody specific for MPV17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPV17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPV17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPV17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.