Human 28S ribosomal protein S35, mitochondrial (MRPS35) ELISA Kit

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for MRPS35. Pseudogenes corresponding to MRPS35 are found on chromosomes 3p, 5q, and 10q.

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Human 28S ribosomal protein S35, mitochondrial (MRPS35) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRPS35. No significant cross-reactivity or interference between Human MRPS35 and analogues was observed.

This Human 28S ribosomal protein S35, mitochondrial (MRPS35) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS35 in samples. An antibody specific for MRPS35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPS35 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS35 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS35 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.