Human Protein MRVI1 (MRVI1) ELISA Kit

MRVI1 is similar to a putative mouse tumor suppressor gene (Mrvi1) that is frequently disrupted by mouse AIDS-related virus (MRV) . Protein MRVI1, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. This protein is a substrate of cGMP-dependent kinase-1 (PKG1) that can function as a regulator of IP3-induced calcium release. Studies in mouse suggest that MRV integration at Mrvi1 induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus MRVI1 may function as a myeloid leukemia tumor suppressor gene. Several alternatively spliced transcript variants encoding different isoforms have been found for MRVI1, and alternative translation start sites, including a non-AUG (CUG) start site, are used.

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Human Protein MRVI1 (MRVI1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRVI1. No significant cross-reactivity or interference between Human MRVI1 and analogues was observed.

This Human Protein MRVI1 (MRVI1) ELISA Kit employs a two-site sandwich ELISA to quantitate MRVI1 in samples. An antibody specific for MRVI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRVI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRVI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRVI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.