Human Myotubularin-related protein 7 (MTMR7) ELISA Kit

MTMR7 encodes a member of the myotubularin family of tyrosine/dual-specificity phosphatases. Myotubularin-related protein 7 is characterized by four distinct domains that are conserved among all members of the myotubularin family: the glucosyltransferase, Rab-like GTPase activator and myotubularins domain, the Rac-induced recruitment domain, the protein tyrosine phosphatases and dual-specificity phosphatases domain and the suppressor of variegation 3-9, enhancer-of-zeste, and trithorax interaction domain. Myotubularin-related protein 7 dephosphorylates the target substrates phosphatidylinositol 3-phosphate and inositol 1,3-bisphosphate. A pseudogene of MTMR7 is found on chromosome 5.

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Human Myotubularin-related protein 7 (MTMR7) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTMR7. No significant cross-reactivity or interference between Human MTMR7 and analogues was observed.

This Human Myotubularin-related protein 7 (MTMR7) ELISA Kit employs a two-site sandwich ELISA to quantitate MTMR7 in samples. An antibody specific for MTMR7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTMR7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTMR7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTMR7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.