Human NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L) ELISA Kit

MT-ND4L (Mitochondrially Encoded NADH:Ubiquinone Oxidoreductase Core Subunit 4L) is a Protein Coding gene. Diseases associated with MT-ND4L include Leber Optic Atrophy and Mitochondrial Complex I Deficiency. Among its related pathways are Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins. and Metabolism. GO annotations related to this gene include NADH dehydrogenase (ubiquinone) activity and oxidoreductase activity, acting on NAD (P) H. Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity) .

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Human NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L) ELISA Kit has high sensitivity and excellent specificity for detection of Human MT-ND4L. No significant cross-reactivity or interference between Human MT-ND4L and analogues was observed.

This Human NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L) ELISA Kit employs a two-site sandwich ELISA to quantitate MT-ND4L in samples. An antibody specific for MT-ND4L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-ND4L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-ND4L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-ND4L bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.