Human Protein MTO1 homolog, mitochondrial (MTO1) ELISA Kit

MTO1 encodes a mitochondrial protein thought to be involved in mitochondrial tRNA modification. The encoded protein may also play a role in the expression of the non-syndromic and aminoglycoside-induced deafness phenotypes associated with a specific mutation in the mitochondrial 12S rRNA gene. Multiple transcript variants encoding different isoforms have been found for MTO1.

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Human Protein MTO1 homolog, mitochondrial (MTO1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTO1. No significant cross-reactivity or interference between Human MTO1 and analogues was observed.

This Human Protein MTO1 homolog, mitochondrial (MTO1) ELISA Kit employs a two-site sandwich ELISA to quantitate MTO1 in samples. An antibody specific for MTO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.