Human Metaxin-2 (MTX2) ELISA Kit

Metaxin-2 encoded by MTX2 is highly similar to the metaxin 2 protein from mouse, which has been shown to interact with the mitochondrial membrane protein metaxin 1. Because of this similarity, it is thought that the encoded protein is peripherally associated with the cytosolic face of the outer mitochondrial membrane, and that it is involved in the import of proteins into the mitochondrion. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 7.

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Human Metaxin-2 (MTX2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTX2. No significant cross-reactivity or interference between Human MTX2 and analogues was observed.

This Human Metaxin-2 (MTX2) ELISA Kit employs a two-site sandwich ELISA to quantitate MTX2 in samples. An antibody specific for MTX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.