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Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit

This Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL12B.

Product Specifications

Background

MYL12B (Myosin Light Chain 12B) is a Protein Coding gene. Among its related pathways are Semaphorin interactions and Tight junction. GO annotations related to this gene include calcium ion binding. An important paralog of this gene is MYL12A.

CAS Number

9000-83-3,7732-18-5

Product Name Alternative

MYL12B; MLC-B; MRLC2; OTTHUMP00000165808; myosin regulatory light chain 2; myosin regulatory light chain MRCL2; myosin regulatory light chain MRLC2

Gene ID

614055

Accession Number

A4IF97

Reactivity

Human

Tag

MYL12B

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

Please inquire

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYL12B. No significant cross-reactivity or interference between Human MYL12B and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

This Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL12B in samples. An antibody specific for MYL12B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL12B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL12B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL12B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

MYL12B; MLC-B; MRLC2; OTTHUMP00000165808; myosin regulatory light chain 2; myosin regulatory light chain MRCL2; myosin regulatory light chain MRLC2

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Accession Number URL

http://www.uniprot.org/uniprot/A4IF97

Calibration Range

Please inquire

Available Sizes

Frequently Asked Questions

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