Human Myosin light chain 5 (MYL5) ELISA Kit

MYL5 encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. Myosin light chain 5, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Myosin light chain 5 (MYL5) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYL5. No significant cross-reactivity or interference between Human MYL5 and analogues was observed.

This Human Myosin light chain 5 (MYL5) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL5 in samples. An antibody specific for MYL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.