Human Myosin-XV (MYO15A) ELISA Kit

MYO15A encodes an unconventional myosin. Myosin-XV differs from other myosins in that it has a long N-terminal extension preceding the conserved motor domain. Studies in mice suggest that this protein is necessary for actin organization in the hair cells of the cochlea. Mutations in MYO15A have been associated with profound, congenital, neurosensory, nonsyndromal deafness. MYO15A is located within the Smith-Magenis syndrome region on chromosome 17. Read-through transcripts containing an upstream gene and MYO15A have been identified, but they are not thought to encode a fusion protein. Several alternatively spliced transcript variants have been described, but their full length sequences have not been determined.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Myosin-XV (MYO15A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO15A. No significant cross-reactivity or interference between Human MYO15A and analogues was observed.

This Human Myosin-XV (MYO15A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO15A in samples. An antibody specific for MYO15A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO15A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO15A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO15A bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.