Human Myosin-Ie (MYO1E) ELISA Kit

MYO1E encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, Myosin-Ie is distinguished by a long tail domain that is involved in crosslinking actin filaments. Myosin-Ie localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in MYO1E are the cause of focal segmental glomerulosclerosis-6. MYO1E has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17.

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Human Myosin-Ie (MYO1E) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO1E. No significant cross-reactivity or interference between Human MYO1E and analogues was observed.

This Human Myosin-Ie (MYO1E) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1E in samples. An antibody specific for MYO1E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO1E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO1E is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO1E bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.