Human Myosin-VIIa (MYO7A) ELISA Kit

MYO7A is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain, an actin-binding domain, a neck domain that interacts with other proteins, and a tail domain that serves as an anchor. MYO7A ncodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness, reduced vestibular function, and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants.

MYO7A; DFNA11; DFNB2; MYOVIIA; MYU7A; NSRD2; USH1B; deafness; autosomal dominant 11; deafness; autosomal recessive 2; myosin VIIA (Usher syndrome 1B (autosomal recessive; severe) )

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Myosin-VIIa (MYO7A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO7A. No significant cross-reactivity or interference between Human MYO7A and analogues was observed.

This Human Myosin-VIIa (MYO7A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO7A in samples. An antibody specific for MYO7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO7A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO7A bound in the initial step. The color development is stopped and the intensity of the color is measured.

MYO7A; DFNA11; DFNB2; MYOVIIA; MYU7A; NSRD2; USH1B; deafness; autosomal dominant 11; deafness; autosomal recessive 2; myosin VIIA (Usher syndrome 1B (autosomal recessive; severe) )

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.