Human Myosin-VIIb (MYO7B) ELISA Kit

Myosin-VIIb encoded by MYO7B is found in brush border microvilli of epithelial cells in the intestines and kidneys. Myosin-VIIb is involved in linking protocadherins to the actin cytoskeleton and is essential for proper microvilli function. This protein aids in the accumulation of intermicrovillar adhesion components such as harmonin and ANKS4B, and this accumulation is necessary for normal brush border action.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Myosin-VIIb (MYO7B) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO7B. No significant cross-reactivity or interference between Human MYO7B and analogues was observed.

This Human Myosin-VIIb (MYO7B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO7B in samples. An antibody specific for MYO7B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO7B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO7B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO7B bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.