Human Myosin-IXb (MYO9B) ELISA Kit

MYO9B encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. Myosin-IXb represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9 (MYH9) . Myosin-IXb has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. Myosin-IXb complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. Myosin-IXb also has rho-GTPase activity. Polymorphisms in MYO9B are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for MYO9B.

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Human Myosin-IXb (MYO9B) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO9B. No significant cross-reactivity or interference between Human MYO9B and analogues was observed.

This Human Myosin-IXb (MYO9B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO9B in samples. An antibody specific for MYO9B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO9B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO9B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO9B bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.