Human NEDD4-binding protein 1 (N4BP1) ELISA Kit

N4BP1 (NEDD4 Binding Protein 1) is a Protein Coding gene. GO annotations related to this gene include RNA binding. An important paralog of this gene is KHNYN. Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCHs substrates and impairing ubiquitination of substrates (By similarity) .

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human NEDD4-binding protein 1 (N4BP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human N4BP1. No significant cross-reactivity or interference between Human N4BP1 and analogues was observed.

This Human NEDD4-binding protein 1 (N4BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate N4BP1 in samples. An antibody specific for N4BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyN4BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for N4BP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of N4BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.